Collagen as a Nanoization Strategy for Enhancing Natural Antioxidants in Cancer Treatment
Keywords:
Collagen, Nanoization, nanoscale resveratrol, cancerAbstract
The purpose of this research was to determine whether nanoscale resveratrol (RSV), collagen added to RSV, is more effective than regular RSV. Collagen is a sturdy emulsifier made in the human body (Cherng et al., 2013). RSV is an antioxidant which has anti-inflammatory properties (Cherng et al., 2013), allowing it to reduce reactive oxygen species (ROS) levels, which can induce cancer when in excess (Arnarson, 2019). However, RSV is not absorbed easily by the human body since it is excreted easily, making it less effective (Cherng et al., 2013). This research paper was focused on overcoming the low bioavailability of RSV through reducing particle size. The concept behind this is nanotechnology, using technology to study 1 to 100 nanometer structures (Bayda et al., 2019). Smaller RSV particles have increased surface area, which dissolves faster in the bloodstream (Deng et al., 2012). The hypothesis is that RSV particles would decrease by 8 times the volume, while the ROS levels in 3T3L1 cells would decrease with nanoscale RSV by 50%. The particle size distribution of RSV was measured with a spectrophotometer, while the ROS levels in 3T3L1 cells were measured by ELISA. The collagen-RSV solution had a greater peak than the regular RSV solution, indicating the particle size of RSV decreased after adding collagen. Also, the nanoscale RSV could reduce ROS levels more than regular RSV. This evidence supports the hypothesis that nanoscale RSV is effective in reducing ROS levels, and is smaller than regular RSV.
References or Bibliography
Arnarson, A. (2019, July 12). Antioxidants explained in simple terms. Healthline. https://www.healthline.com/nutrition/antioxidants-explained
Bai, Y., Mao, Q.-Q., Qin, J., Zheng, X.-Y., Wang, Y.-B., Yang, K., Shen, H.-F., & Xie, L.-P. (2010). Resveratrol induces apoptosis and cell cycle arrest of human T24 bladder cancer cells in vitro and inhibits tumor growth in vivo. Cancer Science, 101(2), 488–493. https://doi.org/10.1111/j.1349-7006.2009.01415.x
Bayda, S., Adeel, M., Tuccinardi, T., Cordani, M., & Rizzolio, F. (2019). The History of Nanoscience and Nanotechnology: From Chemical–Physical Applications to Nanomedicine. Molecules, 25(1), 112. https://doi.org/10.3390/molecules25010112
Chen, Z.-H. . (2004). Resveratrol inhibits TCDD-induced expression of CYP1A1 and CYP1B1 and catechol estrogen-mediated oxidative DNA damage in cultured human mammary epithelial cells. Carcinogenesis, 25(10), 2005–2013. https://doi.org/10.1093/carcin/bgh183
Cherng, J.-H., Chang, S.-J., Yeh, J.-Z., Hung, Y.-W., Lai, H.-C., Liou, N.-H., Li, C.-H., Tsao, C.-A., & Liu, C.-C. (2013). Development of fabrication technique in nano-scale resveratrol by collagen. International Journal of Nanotechnology, 10(10-11), 984–995. https://doi.org/10.1504/ijnt.2013.058124
Deng, Y., Sun, J., Wang, F., Sui, Y., She, Z., Zhai, W., & Wang, C. (2012). Effect of particle size on solubility, dissolution rate, and oral bioavailability: evaluation using coenzyme Q10 as naked nanocrystals. International Journal of Nanomedicine, 2012(7), 5733. https://doi.org/10.2147/ijn.s34365
Deshmukh, S. N., Dive, A. M., Moharil, R., & Munde, P. (2016). Enigmatic insight into collagen. Journal of Oral and Maxillofacial Pathology : JOMFP, 20(2), 276–283. https://doi.org/10.4103/0973-029X.185932
García, P., Phyllissa Schmiedlin-Ren, Mathias, J. S., Tang, H., Christman, G. M., & Zimmermann, E. M. (2012). Resveratrol causes cell cycle arrest, decreased collagen synthesis, and apoptosis in rat intestinal smooth muscle cells. American Journal of Physiology-Gastrointestinal and Liver Physiology, 302(3), G326–G335. https://doi.org/10.1152/ajpgi.00083.2011
Gartel, Andrei L, & Tyner, A. L. (2002). The role of the cyclin-dependent kinase inhibitor p21 in apoptosis 1. Molecular Cancer Therapeutics, 1(8), 639–649. https://aacrjournals.org/mct/article/1/8/639/233906/The-Role-of-the-Cyclin-dependent-Kinase-Inhibitor#
Giordo, R., Zinellu, A., Eid, A. H., & Pintus, G. (2021). Therapeutic potential of resveratrol in Covid-19-associated hemostatic disorders. Molecules, 26(4), 856. https://doi.org/10.3390/molecules26040856
Hashim, P., Ridzwan, M. S., Bakar, J., & Hashim, D. M. (2015). Collagen in food and beverage industries. International Food Research Journal, 22(1), 1–8. https://www.proquest.com/openview/9ea164bebbc97f8c96510a292f3e337c/1?pq-origsite=gscholar&cbl=816390
Khan, I., Saeed, K., & Khan, I. (2017). Nanoparticles: Properties, applications and toxicities. Arabian Journal of Chemistry, 12(7), 908–931. https://doi.org/10.1016/j.arabjc.2017.05.011
Ko, J.-H., Sethi, G., Um, J.-Y., Shanmugam, M. K., Arfuso, F., Kumar, A. P., Bishayee, A., & Ahn, K. S. (2017). The Role of Resveratrol in Cancer Therapy. International Journal of Molecular Sciences, 18(12), 2589. https://doi.org/10.3390/ijms18122589
Koushki, M., Amiri-Dashatan, N., Ahmadi, N., Abbaszadeh, H.-A., & Rezaei-Tavirani, M. (2018). Resveratrol: A miraculous natural compound for diseases treatment. Food Science & Nutrition, 6(8), 2473–2490. https://doi.org/10.1002/fsn3.855
Nakamura, H., & Takada, K. (2021). Reactive oxygen species in cancer: Current findings and future directions. Cancer science, 112(10), 3945–3952. https://doi.org/10.1111/cas.15068
National Cancer Institute. (2020, September 25). Cancer Statistics. National Cancer Institute; Cancer.gov. https://www.cancer.gov/about-cancer/understanding/statistics
R. Neves, A., Lucio, M., L.C. Lima, J., & Reis, S. (2012). Resveratrol in medicinal chemistry: A critical review of its pharmacokinetics, drug-delivery, and membrane interactions. Current Medicinal Chemistry, 19(11), 1663–1681. https://doi.org/10.2174/092986712799945085
Sharma, S., Stutzman, J. D., Kelloff, G. J., & Steele, V. E. (1994). Screening of potential chemopreventive agents using biochemical markers of carcinogenesis. Cancer Research, 54(22), 5848–5855. https://pubmed.ncbi.nlm.nih.gov/7954413/
Taniguchi, N. (1974). On the Basic Concept of Nanotechnology. Proceeding of the ICPE, 18–23. https://www.scirp.org/(S(vtj3fa45qm1ean45vvffcz55))/reference/ReferencesPapers.aspx?ReferenceID=1973088
Wang, H. (2021). A Review of the Effects of Collagen Treatment in Clinical Studies. Polymers, 13(22), 3868. https://doi.org/10.3390/polym13223868
Zeng, G., Fang, Z., Jin, L., Luo, S., & Zhang, P. (2013). Resveratrol-Mediated Reduction of Collagen by Inhibiting Proliferation and Producing Apoptosis in Human Hypertrophic Scar Fibroblasts. Bioscience, Biotechnology, and Biochemistry, 77(12), 2389–2396. https://doi.org/10.1271/bbb.130502
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