Identifying the difference in bacterial composition and life history strategies between high versus low nutrient soils
DOI:
https://doi.org/10.47611/jsrhs.v11i3.3271Keywords:
Bacteria, Wood-chips, Life-history strategy, FertilizerAbstract
In this study, we aim to identify bacterial composition in soils treated with and without wood chips, as well as high levels and low levels of nitrogen. In order to do this, we used both culture dependent methods including plating, observing, and GenIII plates; as well as culture independent methods which include microbiome sequencing and EcoPlates. By doing this, we concluded that the bacterial communities differ between unamended and wood-chipped treated soils. Multiple differences were identified: rate of carbon consumption, bacterial diversity, rRNA gene copy number, and colony morphologies. Identifying the bacteria in wood-chipped soils will allow us to understand their effect on biogeochemical cycles, plant pathogens, and relationship with the trees (symbiotic/pathogen). Additionally, understanding the effect lower nitrogen levels have on the bacterial composition of soils can lead to reduction of synthetic fertilizer use, therefore lessening the environmental impact of nitrogen.
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