Effectiveness of AgNp Coatings on a PyC Surface in a Staph. Epidermidis-Infused Fluid Flow Model

Abstract

There is a medical need for a more effective heart valve coating that reduces both thrombotic potential and biofilm formation. The antimicrobial properties of silver nanoparticles have placed them under consideration for this application. The purpose of this study is to test the effectiveness of varying concentrations of silver nanoparticle (AgNP) coatings on a pyrolytic surface in a Staphylococcus epidermidis-infused fluid flow model. I hypothesized that as the concentration of the AgNP coating is increased, then there will be a decrease in the Staphylococcus epidermidis growth on the PyC surface because of the hydrophobic and antibiotic properties of silver nanoparticles. I produced a fluid flow model and tested the practicality of different concentrations of AgNps while running the fluid flow model by swabbing the plate for bacteria and incubating the bacteria for seven days. I found that the highest concentration of silver nanoparticles created the highest level of bacterial resistance and the lowest level of fluid retention when inside the model.  Through these experiments, the unique properties of AgNp have been utilized to effectively create a more effective heart valve coating that both reduces the need for further medication after implantation, as well as the risk of biofilm formation on the prosthetic valve.