CRISPR as a Potential Technique to Reduce Suicide Risk

Authors

  • Hidemi Zamora San Ignacio de Recaldo School
  • Javier Cornejo Mentor, San Ignacio de Recalde School

DOI:

https://doi.org/10.47611/jsrhs.v10i3.2240

Keywords:

CRISPR, suicide, suicide gene, gene editing, gene knockout, suicide risk, suicide prevention

Abstract

As suicide is the nineteenth leading cause of death worldwide, it is important to focus on discovering ways to reduce the risk of suicide-related death as much as possible. With CRISPR starting to become increasingly popular over the past few years, this gene editing technique has been used to study how to edit, turn off, or knock out multiple parts of the genome. However, research on genes related to diseases as cystic fibrosis or Alzheimer’s disease has been mainly prioritized and, even though they are of high importance as well, important issues such as suicide have been left into oblivion.

Four genes have been proven to be key in influencing suicide risk, showing that not only environmental factors account for an increased possibility of death by this cause. Therefore, gene editing techniques such as CRISPR could be applied in order to knock out those genes and reduce this risk. This research used Synthego’s guide RNA design tool to predict how the use of CRISPR can be helpful in knocking out those four suicide-related genes and, consequently, in preventing suicide. The top-ranked guide RNAs for each gene were used, showing the best results possible and with the least number of off-targets, which, in turn, demonstrates the effectiveness of CRISPR as a potential technique to reduce the number of suicide-related deaths worldwide.

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References or Bibliography

American Foundation for Suicide Prevention. (2019). Risk factors, protective factors, and warning signs. American Foundation for Suicide Prevention. https://afsp.org/risk-factors-protective-factors-and-warning-signs

Bekris, L., Tsuang, D., Peskind, E., Yu, C., Montine, T., Zhang, J., Zabetian, C., & Leverenz, J. (2015). Cerebrospinal fluid Aβ42 levels and APP processing pathway genes in Parkinson’s disease. Movement Disorders, 30(7), 936–944. https://doi.org/10.1002/mds.26172

Bondy, B., Buettner, A., & Zill, P. (2006). Genetics of suicide. Molecular Psychiatry, 11(4), 336–351. https://doi.org/10.1038/sj.mp.4001803

Centers for Disease Control and Prevention. (2021). Facts About Suicide. Centers for Disease Control and Prevention. https://www.cdc.gov/suicide/facts/index.html

Coon, H., Darlington, T., DiBlasi, E., Callor, B., Ferris, E., Fraser, A., Yu, Z., William, N., Das, S., Crowell, S., Chen, D., Anderson, J., Klein, M., Jerominski, L., Cannon, D., Shabalin, A., Docherty, A., Williams, M., Smith, K., & Keeshin, B. (2020). Genome-wide significant regions in 43 Utah high-risk families implicate multiple genes involved in risk for completed suicide. Molecular Psychiatry, 25, 3077–3090. https://doi.org/10.1038/s41380-018-0282-3

Doench, J. (2017). Am I ready for CRISPR? A user’s guide to genetic screens. Nature Reviews Genetics, 19(2), 67–80. https://doi.org/10.1038/nrg.2017.97

Dwivedi, Y. (2012). The Neurobiological Basis of Suicide. CRC Press.

Ibrahim, O., & Umar, A. (2018). CRISPR Technology Advantages, Limitations and Future Direction. Journal of Biomedical and Pharmaceutical Sciences, 1(2). https://www.hilarispublisher.com/open-access/crispr-technology-advantages-limitations-and-future-direction.pdf

Karaca, E., Harel, T., Pehlivan, D., Jhangiani, S., Gambin, T., Coban, Z., Gonzaga, C., Erdin, S., Bayram, Y., Campbell, I., Hunter, J., Atik, M., Van, H., Yuan, B., Wiszniewski, W., Isikay, S., Yesil, G., Yuregir, O., Tug, S., & Aslan, H. (2015). Genes that Affect Brain Structure and Function Identified by Rare Variant Analyses of Mendelian Neurologic Disease. Neuron, 88(3), 499–513. https://doi.org/10.1016/j.neuron.2015.09.048

Leu, J., Chang, S., Mu, C., Chen, M., & Yan, B. (2018). Functional domains of SP110 that modulate its transcriptional regulatory function and cellular translocation. Journal of Biomedical Science, 25(1). https://doi.org/10.1186/s12929-018-0434-4

McGuffin, P., Marušič, A., & Farmer, A. (2001). What Can Psychiatric Genetics Offer Suicidology? Crisis, 22(2), 61–65. https://doi.org/10.1027//0227-5910.22.2.61

Naeem, M., Majeed, S., Hoque, M., & Ahmad, I. (2020). Latest Developed Strategies to Minimize the Off-Target Effects in CRISPR-Cas-Mediated Genome Editing. Cells, 9(7). https://doi.org/10.3390/cells9071608

Nowicki, S. (2017). Holt McDougal Biology. Houghton Mifflin Harcourt Publishing Company.

Pedersen, N., & Fiske, A. (2010). Genetic influences on suicide and nonfatal suicidal behavior: Twin study findings. European Psychiatry, 25(5), 264–267. https://doi.org/10.1016/j.eurpsy.2009.12.008

Redman, M., King, A., Watson, C., & King, D. (2016). What is CRISPR/Cas9? Archives of Disease in Childhood - Education & Practice Edition, 101(4), 213–215. https://doi.org/10.1136/archdischild-2016-310459

Synthego. (2021a). Guide RNA for AGBL2 gene. Synthego. https://design.synthego.com/#/validate/results?genome=homo_sapiens_gencode_26_primary&nuclease=cas9&guide=UGCCAAUUUUUAGCUCAGAG

Synthego. (2021b). Guide RNA for APH1B gene. Synthego. https://design.synthego.com/#/validate/results?genome=homo_sapiens_gencode_26_primary&nuclease=cas9&guide=UCUUGCCAUGAACCAAACAA

Synthego. (2021c). Guide RNA for SP110 gene. Synthego. https://design.synthego.com/#/validate/results?genome=homo_sapiens_gencode_26_primary&nuclease=cas9&guide=UACAGGGAUCAAAUUUCUAC

Synthego. (2021d). Guide RNA for SUCLA2 gene. Synthego. https://design.synthego.com/#/validate/results?genome=homo_sapiens_gencode_26_primary&nuclease=cas9&guide=CCCAGAACCUAGAAAGAUUG

Völgyi, K., Gulyássy, P., Háden, K., Kis, V., Badics, K., Kékesi, K., Simor, A., Györffy, B., Tóth, E., Lubec, G., Juhász, G., & Dobolyi, A. (2015). Synaptic mitochondria: A brain mitochondria cluster with a specific proteome. Journal of Proteomics, 120, 142–157. https://doi.org/10.1016/j.jprot.2015.03.005

World Health Organization. (2021). Suicide. World Health Organization. https://www.who.int/news-room/fact-sheets/detail/suicide

Published

11-07-2021

How to Cite

Zamora, H., & Cornejo, J. (2021). CRISPR as a Potential Technique to Reduce Suicide Risk. Journal of Student Research, 10(3). https://doi.org/10.47611/jsrhs.v10i3.2240

Issue

Section

HS Research Articles