Inhibitory effect of Garlic oil, Clove oil and Thyme oil on Micrococcus Luteus and Staphylococcus epidermidis.

Authors

  • Fadi Al-Shoaibi Coulsdon Sixth Form College
  • Surya Benedicts Coulsdon Sixth Form College

DOI:

https://doi.org/10.47611/jsrhs.v10i3.1534

Keywords:

Micrococcus luteus, staphylococcus epidermidis, coefficient of variation

Abstract

This study is aimed to investigate the antimicrobial activity of Garlic, Thyme and Clove essential oils against Micrococcus luteus and Staphylococcus epidermidis. Using natural oils to fight the bacteria will limit the usage of antibiotics, reducing the probability of antibiotic resistance which is a global increasing problem. Also, it will eliminate antibiotic side-effects such as vomiting, diarrhoea and abdominal pain, Which occurs around 1 in 10 people (NHS, 2019). Antibiotics also have an effect the biofilm layer, causing a decrease in immunity. Micrococcus luteus has shown no growth in the trail run when the extracts where undiluted, nor in the main investigation when dilutions took place. This reveals the susceptibility of the bacteria to the following essential oils. Staphylococcus epidermidis has shown to be more resistant than micrococcus luteus. The oils however have produced a diameter of inhibition zone (DIZ), which means the oils are effective. Clove essential oil has produced the smallest inhibition zones in all concentrations carried out, suggesting that it’s the least effective extract. Thyme oil and clove oil have produced similar results; however, Thyme has shown a stronger antimicrobial effect at the 30 and 40% concentrations, whereas garlic has shown a stronger effect using the 20% concentration which has the highest coefficient of variation at 32.00% suggesting that it’s the least precise result.  These results indicate that these essential oils have strong antimicrobial properties suggesting a potential clinical relevance in tackling bacteria.

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References or Bibliography

Ionescu, (2017), ‘Herbal products an alternative to antibiotics’, IntechOpen. Available at: https://www.intechopen.com/books/bacterial-pathogenesis-and-antibacterial-control/are-herbal-products-an-alternative-to-antibiotics-

Cui, Zhang, Li, Lin, (2018), ‘antimicrobial mechanism of clove oil on Listeria monocytogenes’, Science direct. Available at:https://www.sciencedirect.com/science/article/abs/pii/S0956713518303360

Wickham Laboratories, (no date), fact sheet: Micrococcus luteus. Available at:https://wickhamlabs.co.uk/technical-resource-centre/fact-sheet-micrococcus-luteus/

Science daily, (2013), ‘super sunscreen from fjord bacteria’. Available at:https://www.sciencedaily.com/releases/2013/08/130806091556.htm

Kocur, M., Klosss, W. E., Schliefere K, (2006), ‘Micrococcus’, Microbe Wiki. Available at: https://microbewiki.kenyon.edu/index.php/Micrococcus

Penn Medicine, (2020), ‘what you need to know about being immunocompromised during Covid 19’. Available at:https://www.pennmedicine.org/updates/blogs/health-and-wellness/2020/may/what-it-means-to-be-immunocompromised

Khan, Baig, Mehboob, (2016), ‘Nosocomial infections: Epidemiology, prevention, control and surveillance’, Science direct. Available at:https://www.sciencedirect.com/science/article/pii/S2221169116309509

Nguyen, (2018), ‘pathogen recruits good bacteria on skin to initiate infections’, c&en. Available at:https://cen.acs.org/biological-chemistry/infectious-disease/Pathogen-recruits-bacteria-skin-initiate/96/i30

Artis micropia, (no date), ‘staphylococcus epidermidis’. Available at:https://www.micropia.nl/en/discover/microbiology/staphylococcus-epidermidis/

Otto, (2010), ‘Staphylococcus epidermidis-the “accidental” pathogen’, PMC. Available at: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2807625/

Palraj, Wilson, (2016), ‘Prosthetic valve Endocarditis’, Science direct. Available at:https://www.sciencedirect.com/topics/medicine-and-dentistry/prosthetic-valve-endocarditis

Science direct, (2015), ‘Staphylococcus epidermidis’. Available at:https://www.sciencedirect.com/topics/medicine-and-dentistry/staphylococcus-epidermidis

Encyclopaedia Britannica, (no date), ‘Clove’, Britannica. Available at:https://www.britannica.com/plant/clove

Khalilzadeh, Hazrati, Gholamreza, (2016), ‘Effects of topical systemic administration of Eugenia caryophyllata buds essential oil on corneal anaesthesia and analgesia’, PMC. Available at:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5022377/

Packyanathan, Prakasam, (2017), ‘antibacterial effect of clove oil against clinical strains of Escherichia coli’, Journal of pharmaceutical sciences and research. Available at:https://www.jpsr.pharmainfo.in/Documents/Volumes/vol9Issue07/jpsr09071734.pdf

Singletary, (2016), ‘Thyme’, Nutrition today. Available at:https://journals.lww.com/nutritiontodayonline/fulltext/2016/01000/thyme__history,_applications,_and_overview_of.10.aspx

Nzeako, Al-Kharousi, Al-Mahrooqui, (2016), ‘antimicrobial activities of clove and thyme extracts’, PMC. Available at:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3074903/

Ejigu, (2016), ‘Anti-bacterial effect of garlic against clinical isolates of S. Aureus and E. Coli from patients attending Hawassa referral hospital, Ethiopia’, Research gate. Available at:https://www.researchgate.net/publication/312231138_Anti-Bacterial_Effect_of_Garlic_Allium_sativum_against_Clinical_Isolates_of_Staphylococcus_aureus_and_Escherichia_coli_from_Patients_Attending_Hawassa_Referral_Hospital_Ethiopia

Abiy, Berhe, (2016), ‘Anti-bacterial effect of garlic against clinical isolates of S. Aureus and E. Coli from patients attending Hawassa referral hospital, Ethiopia’, Journal of infectious Diseases and Treatment. Available at:https://infectious-diseases-and-treatment.imedpub.com/antibacterial-effect-of-garlic-allium-sativumagainst-clinical-isolates-of-staphylococcusaureus-and-escherichia-coli-from-patientsa.php?aid=17777#2

Leontiev, Hohaus, claus, Gruhlke, Slusarenko, (2018), ‘A comparison of antibacterial and anti fungal activities of Thiosulfinate analogues of Allicin’, Nature. Available at:https://www.nature.com/articles/s41598-018-25154-9

NHS, (2019), Side effects. Available at: https://www.nhs.uk/conditions/antibiotics/side-effects/

Chan, Kong, Yee, Chua, Loo, (2012), ‘Rosemary and Sage outperformed six other culinary herbs…’ , International journal of Biotechnology and wellness industries. Available at:https://www.lifescienceglobal.com/pms/index.php/ijbwi/article/view/232

Verhoef, Fleer, (1983), ‘Staphylococcus epidermidis endocarditis and staphylococcus epidermidis infection in an intensive care unit’, pubMed. Available at:https://pubmed.ncbi.nlm.nih.gov/6589762/

Science direct, (2019), ‘Staphylococcus epidermidis’. Available at:https://www.sciencedirect.com/topics/medicine-and-dentistry/staphylococcus-epidermidis

Buttner, Mack, Rohde, (2015), ‘Structural basics of staphylococcus epidermis biofilm formation: mechanisms and molecular interactions’, frontiers. Available at:https://www.frontiersin.org/articles/10.3389/fcimb.2015.00014/full#B119

Holland and Barrett, (no date), ‘about us’ . Available at:https://www.hollandandbarrett.com/info/who-we-are/

Published

10-10-2021

How to Cite

Al-Shoaibi, F., & Benedicts, S. (2021). Inhibitory effect of Garlic oil, Clove oil and Thyme oil on Micrococcus Luteus and Staphylococcus epidermidis . Journal of Student Research, 10(3). https://doi.org/10.47611/jsrhs.v10i3.1534

Issue

Section

HS Research Articles