The effects of cleaning solutions on bacteria concentration on hospital wheelchairs


  • Erin Knapp Mentor High School



bacteria, HAIs, Hospital-acquired infections, nosocomial infections, cleaning, patient transport, cleaning solutions, bacteria concentration, hospital transportation, wheelchairs, hospital wheelchairs


To what extent does the concentration of bacteria located on hospital wheelchairs vary based on the cleaning solution used between patient transport? The purpose of this study was to investigate the cause of HAIs [Hospital Acquired Infections] in hospital environments and determine the best cleaning method of wheelchairs in hospitals. The method was to swab the seats and handles of wheelchairs before and after cleaning them while wearing gloves in comparison to no gloves with varying strengths of cleaning solutions, plating the swab, and allowing the bacteria to be incubated and grow in an incubator. Then, every day for 10 days, plates were measured on a piece of graph paper to record growth. A one-way ANOVA was calculated for the effectiveness of the various cleaners and a p-value of 0.6597 was achieved and a t-test was performed to compare the values of gloves versus no gloves and a p-value of 0.2216 was calculated. The p-values were not significant at 0.05. Overall, wearing gloves while cleaning showed to have a slight positive impact on the overall cleanliness but was not statistically significant, and the highest strength wipes inhibited bacterial growth most often. Hospital protocol for the cleansing of wheelchairs between patient use should be updated to the use of gloves and high strength wipes to stop the spread of bacterial infections to patients.


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References or Bibliography

Arefian, H., Hagel, S., Fischer, D., Scherag, A., Brunkhorst, F. M., Maschmann, J., & Hartmann, M. (2019). Estimating extra length of stay due to healthcare-associated infections before and after implementation of a hospital-wide infection control program. PLoS ONE, 14(5), 1–11. .

Bello, A., Quinn, M. M., Perry, M. J., & Milton, D. K. (2009). Characterization of occupational exposures to cleaning products used for common cleaning tasks--a pilot study of hospital cleaners. Environmental health : a global access science source, 8, 11.

“CDC Healthcare-associated Infections Data Portal.” (2020, January 02). Retrieved April 18, 2020, from

Dancer, S. J., White, L., & Robertson, C. (2008). Monitoring environmental cleanliness on two surgical wards. International Journal of Environmental Health Research, 18(5), 357–364. doi: 10.1080/09603120802102465

Drexler, M. (2010). What You Need to Know About Infectious Disease. Washington (DC): National Academies Press (US);. How Infection Works.

Dziewa, A., Ksykiewicz-Dorota, A., Kos, M., & Drop, B. (2015). Nurse care quality and hospital-acquired infections: adhering to aseptic techniques. Polish Journal of Public Health, 125(3), 133–136.

Fathizadeh, H., Maroufi, P., Momen-Heravi, M., Dao, S., Köse, Ş., Ganbarov, K., Pagliano, P., Esposito, S., & Kafil, H. S. (2020). Protection and disinfection policies against SARS-CoV-2 (COVID-19). Le infezioni in medicina, 28(2), 185–191.

Han, J. H., Sullivan, N., Leas, B. F., Pegues, D. A., Kaczmarek, J. L., & Umscheid, C. A. (2015). Cleaning Hospital Room Surfaces to Prevent Health Care–Associated Infections. Annals of Internal Medicine, 163(8), 598. doi: 10.7326/m15-1192

Ioannou, C. J., Hanlon, G. W., & Denyer, S. P. (2006). Action of Disinfectant Quaternary Ammonium Compounds against Staphylococcus aureus. Antimicrobial Agents and Chemotherapy, 51(1), 296-306. doi:10.1128/aac.00375-06

Ledford, H. (2008, November 13). How does bleach bleach? Nature. doi:

Lopez-Alcalde, J., Conterno, L. O., Mateos-Mazón, M., Guevara-Eslava, M., Job-Neto, F., & Solà, I. (2008). Gloves, gowns and masks for reducing the transmission of meticillin-resistant Staphylococcus aureus (MRSA) in the hospital setting. Cochrane Database of Systematic Reviews. doi:10.1002/14651858.cd007087

Moore D. L. (2018). Infection prevention and control in paediatric office settings. Paediatrics & child health, 23(8), 547–548.

Muhonja, C. N., Makonde, H., Magoma, G., & Imbuga, M. (2018). Biodegradability of polyethylene by bacteria and fungi from Dandora dumpsite Nairobi-Kenya. PloS one, 13(7), e0198446.

Phenolics. (2020). Retrieved from

Revelas A. (2012). Healthcare - associated infections: A public health problem. Nigerian medical journal : journal of the Nigeria Medical Association, 53(2), 59–64. doi:10.4103/0300-1652.103543

Rothan, H. A., & Byrareddy, S. N. (2020). The epidemiology and pathogenesis of coronavirus disease (COVID-19) outbreak. Journal of autoimmunity, 109, 102433.

Russotto, V., Cortegiani, A., Fasciana, T., Iozzo, P., Raineri, S. M., Gregoretti, C., … Giarratano, A. (2017). What Healthcare Workers Should Know about Environmental Bacterial Contamination in the Intensive Care Unit. BioMed Research International, 2017, 1–7. doi: 10.1155/2017/6905450

Rutala, W. A., & Weber, D. J. (2014). Selection of the Ideal Disinfectant. Infection Control & Hospital Epidemiology, 35(7), 855–865. doi: 10.1086/676877

Siddique, R., Anjaneyulu, K., & Muralidharan, N. P. (2019). Antimicrobial Efficacy of Garlic-Lemon in Comparison with Sodium Hypochlorite against E. faecalis. Journal of Clinical & Diagnostic Research, 13(1), 55–58.

Tebbutt G. M. (1988). Laboratory evaluation of disposable and reusable disinfectant cloths for cleaning food contact surfaces. Epidemiology and infection, 101(2), 367–375.

Tessarolo, F., Caola, I., Fedel, M., Stacchiotti, A., Caciagli, P., Guarrera, G., . . . Nollo, G. (2007). Different experimental protocols for decontamination affect the cleaning of medical devices. A preliminary electron microscopy analysis. Journal of Hospital Infection, 65(4), 326-333. doi:10.1016/j.jhin.2006.10.015

Velvizhi, G., Anupriya, G., Sucilathangam, G., Ashihabegum, M. A., Jeyamuruga, T., & Palaniappan, N. (2012). Wristwatches as the Potential Sources of Hospital-Acquired Infections. Journal of Clinical & Diagnostic Research, 6(5), 807–810. Retrieved from

Vesley, D., Klapes, N. A., Benzow, K., & Le, C. T. (1987). Microbiological evaluation of wet and dry floor sanitization systems in hospital patient rooms. Applied and Environmental Microbiology, 53(5), 1042-1045. doi:10.1128/aem.53.5.1042-1045.1987

Walsh, T. (2008, August). Antimicrobial wipes should not be used on consecutive surfaces. Infectious Disease News, p. 20. Retrieved from

most common healthcare-associated infections. (2014, September 4). Retrieved February 17, 2020, from



How to Cite

Knapp, E. (2020). The effects of cleaning solutions on bacteria concentration on hospital wheelchairs. Journal of Student Research, 9(1).



AP Research Articles